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Current Affairs 2017

Delhi Police chief Alok Verma appointed new CBI Director

The Appointments Committee of the Cabinet has approved the appointment of Shri Alok Kumar Verma as the new CBI Director. His appointment will be for a period of two years from the date of assumption of charge of his office. Shri Alok Kumar Verma, currently Delhi Police commissioner, is a 1979 batch Indian Police Service officer of the Arunachal Pradesh-Goa-Mizoram and Union Territory (AGMUT) cadre.

Employment News

181 Pet PI ASC, C/o 56 APO, www.persmin.nic/dopt.asp invites applications for Tradesman Mate 02 psots (UR-01 post, SC/ST-01) Fireman 06 posts (UR-04, SC/ST-01post, OBC-01) Refer website or Employment News dated 7-13 Jan 2017...

Human Genome

The human genome is the genome of Homo sapiens, which is stored on 23 chromosome pairs. Twenty-two of these are autosomal chromosome pairs, while the remaining pair is sex-determining. The haploid human genome occupies a total of just over 3 billion DNA base pairs. The haploid human genome contains ca. 23,000 protein-coding genes, far fewer than had been expected before its sequencing. In fact, only about 1.5% of the genome codes for proteins, while the rest consists of non-coding RNA genes, regulatory sequences, introns, and (controversially named) "junk" DNA

The Human Genome Project (HGP) produced a reference sequence of the euchromatic human genome, which is used worldwide in biomedical sciences.

Genes

Surprisingly, the number of human genes seems to be less than a factor of two greater than that of many much simpler organisms, such as the roundworm and the fruit fly. However, human cells make extensive use of alternative splicing to produce several different proteins from a single gene, and the human proteome is thought to be much larger than those of the aforementioned organisms. Besides, most human genes have multiple exons, and human introns are frequently much longer than the flanking exon.

Human genes are distributed unevenly across the chromosomes. Each chromosome contains various gene-rich and gene-poor regions, which seem to be correlated with chromosome bands and GC-content. The significance of these nonrandom patterns of gene density is not well understood. In addition to protein coding genes, the human genome contains thousands of RNA genes, including tRNA, ribosomal RNA, microRNA, and other non-coding RNA genes.

Regulatory Sequences

The human genome has many different regulatory sequences which are crucial to controlling gene expression. These are typically short sequences that appear near or within genes. A systematic understanding of these regulatory sequences and how they together act as a gene regulatory network is only beginning to emerge from computational, high-throughput expression and comparative genomics studies. Some types of non-coding DNA are genetic "switches" that do not encode proteins, but do regulate when and where genes are expressed.

Identification of regulatory sequences relies in part on evolutionary conservation. The evolutionary branch between the human and mouse, for example, occurred 70–90 million years ago.

So computer comparisons of gene sequences that identify conserved non-coding sequences will be an indication of their importance in duties such as gene regulation.

Another comparative genomic approach to locating regulatory sequences in humans is the gene sequencing of the puffer fish. These vertebrates have essentially the same genes and regulatory gene sequences as humans, but with only one-eighth the "junk" DNA. The compact DNA sequence of the puffer fish makes it much easier to locate the regulatory genes.

ory gene sequences as humans, but with only one-eighth the "junk" DNA. The compact DNA sequence of the puffer fish makes it much easier to locate the regulatory genes

Other DNA

Protein-coding sequences (specifically, coding exons) comprise less than 1.5% of the human genome. Aside from genes and known regulatory sequences, the human genome contains vast regions of DNA the function of which, if any, remains unknown. These regions in fact comprise the vast majority, by some estimates 97%, of the human genome size. Much of this is composed of:

Repeat elements.

Tandem repeat: Tandem repeats occur in DNA when a pattern of two or more nucleotides is repeated and the repetitions are directly adjacent to each other.An example would be:
 A-T-T-C-G-A-T-T-C-G-A-T-T-C-G
in which the sequence A-T-T-C-G is repeated three times.

Interspersed repetitive DNA is found in all eukaryotic genomes. Certain classes of these sequences propagate themselves by RNA mediated transposition, and they have been called retrotransposons.

Transposons

The major difference of class II transposons from retrotransposons is that their transposition mechanism does not involve an RNA intermediate. Class II transposons usually move by a mechanism analogous to cut and paste, rather than copy and paste, using the transposase enzyme. Different types of transposase work in different ways. Some can bind to any part of the DNA molecule, and the target site can therefore be anywhere, while others bind to specific sequences. Transposase makes a staggered cut at the target site producing sticky ends, cuts out the transposon and ligates it into the target site.

Junk DNA

However, there is also a large amount of sequence that does not fall under any known classification. Much of this sequence may be an evolutionary artifact that serves no present-day purpose, and these regions are sometimes collectively referred to as "junk" DNA. There are, however, a variety of emerging indications that many sequences within are likely to function in ways that are not fully understood. Recent experiments using microarrays have revealed that a substantial fraction of non-genic DNA is in fact transcribed into RNA which leads to the possibility that the resulting transcripts may have some unknown function. Also, the evolutionary conservation across the mammalian genomes of much more sequence than can be explained by protein-coding regions indicates that many, and perhaps most, functional elements in the genome remain unknown. The investigation of the vast quantity of sequence information in the human genome whose function remains unknown is currently a major avenue of scientific inquiry.

Author: Dr. Sujata Roy
Assistant Professor,
Biotechnology Department.
Rajalakshmi Engineering College, Chennai

Swarnjayanti Gram Swarozgar Yojana

The Ministry of Rural Development, Government of India has launched a new programme known as "Swarnjayanti Gram Swarozgar Yojana" (SGSY) by restructuring the existing schemes namely : Integrated Rural Development Programme (IRDP)Training of Rural Youth for Self Employment (TRYSEM)Development of Women & Children in Rural Areas (DWCRA)Supply of Improved Toolkits to Rural Artisans (SITRA)Ganga Kalyan Yojana (GKY)Million Wells Scheme (MWS)The Scheme The SGSY Scheme is operative from 1st April 1999 in rural areas of the country. SGSY is holistic Scheme covering all aspects of self-employment such as organization of the poor into Self Help Groups, training, credit, technology, infrastructure and marketing. The scheme will be funded by the financial institutions, Panchayat Raj Institutions, District Rural Development Agencies (DRDAs), Non Government Organisation (NGOs), Technical institutions in the district; will be involved in the process of planning, implementation and monitoring of th ...

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What are the Child Rights?

Who is a Child? Article 1 of the UN Convention on the Rights of the Child says, a child means every human being below the age of eighteen years unless under the law applicable to the child, majority is attained earlier. What is the Convention on the Rights of the Child? The United Nations Convention on the Rights of the Child, often referred to as CRC or UNCRC, is an international convention setting out the civil, political, economic, social and cultural rights of children. Nations that ratify this international convention are bound by it by international law. The Convention- on the Rights of the Child defines basic rights of children covering multiple needs and issues. India endorsed it on December 11, 1992. Following are a few rights in the immediate purview. The right to Education: 50% of Indian children aged 6-18 do not go to school Dropout rates increase alarmingly in class III to V, its 50% for boys, 58% for girls. The right to Expression: Every child has a right to e ...

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History of Indian Cinema

India's first film "Raja Harischandra", a silent movie was first screened at Bombay's (Mumbai) Coronation Theater on May 3, 1913. It was made by Dadasaheb Phalke, the father of Indian Cinema. It was Phalke who introduced India to world cinema at a time when working in films was restricted by social customs. After the success of his film 'Raja Harishchandra', several filmmakers in Bombay and Madras began making silent films. By the mid 1920s, Madras had become the epicenter for all film related activities. Raghupathi Venkaiah Naidu, S S Vasan, A V Meiyappan set up production houses in Madras to shoot Telugu and Tamil films. The silent era came to an end when Ardeshir Irani produced his first talkie, 'Alam Ara' in 1931. If Phalke was the father of Indian cinema, Irani was the father of the talkie. The talkies changed the face of Indian cinema. Apart from looks, the actors not only needed a commanding voice but also singing skills, as music became a defining element in Indian cinema. The ...

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Career Scope in Advertising Industry

Advertising world has led to the emergence of many new roles within the advertising profession. In the new edge of ad world, advertising goes beyond its conventional approach. For the non-conventional and conventional advertising approach, Industry requires well groomed people who can develop innovative and creative new idea. Flexibility and competence is must for the industry as the Ad World is highly creative as well as dynamic. Advertising careers are diverse and can run across a variety of departments, offering positions in the fields of creative departments, production, media, and research. The ultimate goal is to meet the client’s goals of increasing sales as much as possible. Public relations services are often involved with businesses, governments, and institutions and can help them make effective decisions. Students of an advertising school may choose to pursue a career as a: Advertising Media PlannerMedia ResearcherCreative DepartmentCopywriter/IllustratorAccount PlanningPr ...

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